Detection of cell membrane proteins that interact with virulent infectious bursal disease virus.

نویسندگان

  • A Setiyono
  • T Hayashi
  • T Yamaguchi
  • H Fukushi
  • K Hirai
چکیده

To detect the molecules that interact with infectious bursal disease virus (IBDV), the chicken B lymphoblastoid cell line, LSCC-BK3, which is permissive for virulent IBDV infection was investigated. The sodium dodecyl sulfate-solubilized plasma membrane fraction from the cells was subjected to a virus overlay protein binding assay. The IBDV specifically bound to proteins in LSCC-BK3 plasma membranes with molecular weights of 70, 82 and 110 kDa. This is the first report to demonstrate cellular molecules that interact with virulent IBDV.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Crude extract of bacterially expressed, infectious bursal disease virus VPX/VP2 proteins induces protective immune response to the virus in chickens

Infectious bursal disease virus (IBDV) infects young chickens and causes serious lose to the poultry industry, worldwide. Previous attempts using purified bacterially expressed IBDV VP2 failed to elicit a protective immune response to the virus. This study was designed to investigate if the initial expressed protein contained neutralizing epitopes but became nonfunctional during purification st...

متن کامل

Rapid differentiation between very virulent and classical infectious bursal disease viruses isolated in Iran by RT-PCR/REA

Abstract: This study was conducted to characterize infectious bursal disease virus (IBDV) isolates collected from different parts of Iran during 2005-2006. Pooled bursal samples from 49 broiler and layer pullet flocks suspected to IBD infection were collected and processed. A reverse transcriptase-polymerase chain reaction (RT PCR) procedure was used to amplify a VP2 gene fragment (743 bp) from...

متن کامل

Sequence analysis of the VP1 gene in three very virulent Iranian infectious bursal disease virus strains

Infectious bursal disease (IBD) is a highly contagious disease of chickens caused by the infectious bursal disease virus (IBDV). This study was conducted to characterize three IBDV strains from Iran. A reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was used to amplify a 715-bp fragment of the VP1 gene from IBDV strains. Amplified VP1 fragments of the three Iranian IBDV strai...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Journal of veterinary medical science

دوره 63 2  شماره 

صفحات  -

تاریخ انتشار 2001